Brand new personal phrase out-of Blk in B muscle could have been confronted by overview of Blk expression inside peoples thymocytes (10). ? (Fig.1C), step 1 C), a potential aftereffect of Blk deficit to your T-telephone innovation is actually examined. The thymuses of Blk-deficient and you can manage mice had been off equal size, plus the rates out of CD4 and CD8 structure regarding the thymuses and you will spleens away from Blk-deficient rats was similar to into the 129/Sv manage rats (investigation maybe not shown). I and additionally failed to detect any difference between T-cell receptor ??, CD3?, heat-secure antigen, and you may CD69 expression inside the splenic T-cell and you can thymocyte subpopulations out of handle and you can Blk-lacking mice (research not revealed).

Proteins tyrosine phosphorylation.

The part off Blk in BCR-induced signaling was addressed because of the research out of body IgM-mediated tyrosine phosphorylation out of intracellular protein for the filtered splenic B muscle. The latest patterns out of phosphoproteins in whole-cell lysates out-of unstimulated and you can anti-IgM-addressed 129/Sv manage and you will Blk-deficient B muscle had been comparable (Fig. ? (Fig.3A). step 3 Good). Since a deficiency of Blk possess especially inspired this new phosphorylation away from Blk-relevant healthy protein, the brand new phosphorylation away from protein which bind toward SH2 domain name away from Blk (Blk-SH2) try particularly assessed. Continuarea →