The SD area for O. luzonensis maps ranging from eyeless and you may 171M23F to the LG 12 (Tanaka ainsi que al. 2007). We performed then linkage investigation and you can obtained a couple of male recombinants to own this place. That male got a great recombination breakpoint between OluX2-8 and OluX2-twenty five, plus the other got a good breakpoint ranging from OluX3-34 and you can OluX4-six, polishing this new SD part so you’re able to anywhere between OluX2-8 and you will OluX4-6 ( Contour 1A ).

(A) Genetic and real chart of one’s gender-determining regions with the X and Y chromosomes. step one, regular XY male; 2, XX females; 3 and you may 4, recombinant men. Blue line, regions produced from new Y chromosome; purple, from the X chromosome. Red-bar, BAC clones; bluish bar, fosmid clones. Black arrows mean forecast genes. (B) RT–PCR circumstances from PGs (PG1–9) throughout the XX and you may XY muscles trunk area at 0 weeks after hatching. Meters, dimensions marker.

We built a great BAC collection from an enthusiastic XX seafood and you can an excellent fosmid library away from a good YY fish, therefore we made physical charts of your own SD region of the X and you will Y chromosomes. This place is actually covered with a couple of BAC clones (OluBXKN2 and you will OluBXKN1) toward X chromosome along with seven fosmid clones (OluFY13-1, OluFY24-step one, OluFYstep one8-1, OluFY3-1, OluFY8-step 1, OluFY7-step 1, and OluFY29-1) towards Y-chromosome ( Profile 1A ). The entire nucleotide series are determined by playing with shotgun sequencing, except for a repeated part from inside the OluFY3-step one and you will OluBXKN1. Limitation research from each other clones demonstrated that period of the fresh new repetitive region was an identical for chromosomes (data perhaps not revealed). Continuarea →